- Institution: The Biological Resource Centre of the Institut Pasteur
- Acronym: CIP/CRBIP
Institut Pasteur. 25-28 rue du Docteur Roux
- Website: http://www.pasteur.fr/en/public-health/crbip
Dominique CLERMONT/ Mery PINA
Tel.: +33 (0)1 45 68 87 75
Fax: +33 (0)1 40 61 30 07
The Biological Resource Centre of the Institut Pasteur (CRBIP) was established in December 2001, with the aim of gathering and preserving Pasteurian collections of biological materials and associated information, and distributing these materials in accordance to current national and international rules. The CRBIP gathers the Collection of Institut Pasteur (CIP: bacteria and plasmids), Pasteur Cyanobacteria Collection (PCC) and the ICAReB biobank. It has participated in the drafting of the ISO norm in management of culture collections and Biosecurity, and it has also participated in discussions about the establishment of the Nagoya Protocol with the French parliament. At the European level, the CRBIP coordinated the MIRRI infrastructure, together with the DSMZ (German microbial BRC) and CABI (English microbial BRC). The CRBIP also participates in the EMBRIC cluster and, at national level, is involved in the French infrastructure BIOBANQUES and it has coordinated the development of new software for the management of Biological Resource Centers of micro-organisms (mBRCs) with the participation of 9 French mBRCs.
The Collection of the Institut Pasteur (CIP) was founded by Dr Binot that began to preserve strains since 1892. The freeze-drying began in the 1950s in the CIP. For over a century, the CIP can be found on the campus of the Institut Pasteur.
The CIP ensures the maintenance, the enrichment and the distribution of its bacterial collection as well as the diffusion of information on the distributed strains via a web site. More than 15 000 strains are available on the web catalogue, including type strains representative of around 5000 different species, strains containing plasmid, genes of interest, strains having particular properties, etc... Every year, new bacterial strains are added to the collection coming either from researchers of the Institut Pasteur, or from foreign collections or from newly deposited strains after having connection with foreign or French researchers. Strains are preserved in two different places and by two different ways (freeze dried and deep-frozen at - 80°C, or in nitrogen liquid and at - 80°C for strains which cannot be freeze-dried).
The CIP is a member of different official organizations: l’ECCO (European Culture Collections’ Organization – www.eccosite.org), GBRCN (Global Biological Resource Centres Network – www.gbrcn.org) project, CABRI (Common Access to Biotechnological Resources and Information – www.cabri.org and WFCC (World Federation for Culture Collection). The CIP has the IBiSA label (IBiSA: Infrastructures en Biologie Santé et Agronomie created in May 2007).
- Supply of cultures, freeze-dried or on agar
- Supply of high molecular mass DNA
- Whole genome sequencing
- MALDI-TOF mass spectrometry
- Deposit of strains for public access
- Scientific consultancy
The CIP performs identification, characterization and typing of strains, using phenotypic techniques (conventional tests) as well as genomic techniques (sequence analysis of 16S rDNA; MLSA of housekeeping genes; whole genome sequencing). The sequences integrated into the CIP database can be viewed and used for Blast comparison. The CIP develops different research projects that focus on bacterial genomics and proteomics, as well as on strain preservation. Collaborations with different laboratories inside and outside of Institut Pasteur are developed.
The CIP collaborates with Bruker for providing the MALDI-TOF mass spectra of biological material which are not included into the Bruker Database.
Quality Management System
CRBIP is NF S 96-900 certified for all aspects of collection management, from the accessioning of strains, their control upon receipt and after preservation, to preservation, storage and supply of cultures and related data.